Quantitative Determination of Chlorogenic Acid in Xiaoer Jiebiao Oral Liquid by HPLC / 中药新药与临床药理

The content of chlorogenic acid in Xiaoer Jiebiao Oral Liquid was determined by HPLC.ODS column was applied.Mobile phase was methanol_1% acetic acid(25∶75),wavelength of detector was 328nm.Samples were extracted with methanol on ultrasonic vibrator.In this method,the linera range of chlorogenic acid is 0.232 ～ 1.16 ?g,the correlation coefficient is 0.9994 ,and the mean recovery is 98.44%. The results showed that this method is simple,quick and reproduciple.